pISSN : 3058-423X eISSN: 3058-4302
Open Access, Peer-reviewed
pISSN : 3058-423X eISSN: 3058-4302
Open Access, Peer-reviewed
Chin Young Park,Su Chul Kim,Seung Chul Lee,Young Pyo Kim,Young Ho Won
Epub 2016 February 24
Abstract
OBJECTIVE: We examined the candidacidal activity of several disinfectants including ethanol, phenol, povidone-iodine, and methanol which has been used commonly in the hospital. Candidal species tested were Candida albicans, Candida krusei, Candida parapsilosis and Candida guillermondii.
METHODS: All candidial stains were cultured in Sabouraud-dextrose agar (SDA), and the spore numbers of them were measured by a hemocytometer. After candidial suspensions were mixed with disinfectants of different concentration for 30 second, they were washed with physiologic saline to remove disinfectants. The suspensions were adjusted into 1x10(4) cfu/ml and were cultured in SDA at 30 degrees C for 72 hours. Minimal fungicidal concentration (MFC) was defined as the minimum concentration of a disinfectant to effect no growth of candidial strains. Minimal inhibitory concentration (MIC) was defined as the minimum concentration of a disinfectant to effect minimum growth of candidial strains.
RESULTS: Our results showed that MFCs of candidial strains were 30~45% in Ethanol, 40~55% in methanol, 0.025~0.06% in povidon-iodine and 1~2% in phenol. All strains tested were killed by ethanol, methanol and povidone-iodine within 2 min at MIC, except phenol.
CONCLUSION: In conclusion, concentrations of disinfectants generally used in the hospital are sufficient to kill candidial strains. Careful procedure should be done in mycologic studies to eliminate the fungicidal effect of disinfectants.
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